Peracetic Acid Sterilization Test Strips

Best Practices for Use of 3000ppm Peracetic Acid Test Strips

• PPE first: chemical-resistant gloves, splash goggles/face shield, impermeable apron, and appropriate respirator if vapour/aerosol risk exists.
• Collect sample safely: transfer a small, known volume (e.g., 10 mL) into a chemically resistant beaker or bottle inside a fume hood or outdoors. Avoid splashes.
• If expected > strip top range, predilute: e.g., perform a 1:10 dilution (1 part sample + 9 parts water). Test the dilution and multiply the reading by the dilution factor. Use labelled, clean containers.
• Neutralize for handling if required: where local SOPs call for neutralization (e.g., adding a validated neutralizer), perform it per SOP before disposal but do not neutralize prior to reading unless your SOP instructs this (you’ll get a lower reading).
• Test using the strip: dip or add sample to strip per manufacturer timing; read at the end point. If the strip shows “off-scale high,” repeat with a higher dilution.
• Record & act: log ppm, dilution factor, operator, time, location, and actions taken. If readings indicate unsafe residuals, follow SOP (additional rinse, hold, notify safety officer).

Miscellaneous Information

For more information on actual shelf life, see our blog: Test Strip Expiration Dates; Good Today, Dead Tomorrow?

You can compare the chemical structures of peracetic acid (aka peroxyacetic acid), chemical formula C₂H₄O₃, to acetic acid, hydrogen peroxide & most quaternary ammonium compounds using our 3D Molecular Model Builder.

Use our free Sanitizer Dilution Calculator to prepare accurate dilutions to any ppm level for any volume. Some knowledge of the metric system is required.